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PSB and Azospirillum: Biofertilisers for Organic Farms

PSB (Phosphate Solubilising Bacteria) and Azospirillum are two of the most cost-effective biofertilisers available to organic farmers β€” PSB converts locked-up soil phosphorus into plant-available form, while Azospirillum fixes atmospheric nitrogen directly in the root zone, supplying a portion of the crop’s nitrogen requirement from the air. Together, they address two of the three primary soil nutrient requirements (N and P) through biological means. At β‚Ή50–100 per kg of commercial product and application rates of 2–2.5 kg/acre for soil treatment, a season’s biofertiliser programme costs β‚Ή200–500/acre vs β‚Ή2,000–5,000/acre for equivalent NPK chemical inputs. The catch: these are living organisms, and effectiveness depends entirely on proper application β€” correct timing, compatible soil conditions, and not mixing with fungicides.

20–30 kg N/acre

Nitrogen fixed by Azospirillum annually in active soil β€” equivalent to 40–60 kg urea savings in supportive conditions

PSB unlocks 30–50%

Of soil phosphorus that was previously locked in insoluble forms β€” reducing need for added phosphate inputs

5 g/kg seed

Standard seed treatment rate for both PSB and Azospirillum β€” coat moist seeds before sowing

Do not mix with copper

Both PSB and Azospirillum are killed by copper fungicides and chemical pesticides β€” apply separately

What Is PSB and How Does It Work?

Phosphate Solubilising Bacteria (primarily Bacillus megaterium, Pseudomonas striata, and related species) secrete organic acids β€” gluconic acid, citric acid β€” that dissolve insoluble tricalcium phosphate, iron phosphate, and aluminium phosphate compounds in the soil, converting them to plant-available orthophosphate. In most Indian soils, 70–80% of applied phosphorus becomes locked in insoluble forms within days of application. PSB essentially unlocks this phosphorus bank.

Where phosphorus locking is most severe:

  • Red laterite soils (common in Karnataka, Tamil Nadu) β€” high iron content locks phosphorus as iron phosphate
  • Acid soils (pH below 6.0) β€” aluminium and iron phosphate precipitation
  • Alkaline black soils β€” calcium phosphate precipitation

PSB effectiveness is highest when:

  • Organic matter is above 1% (microbes need carbon to metabolise)
  • Soil pH is 6.5–7.5 (extreme pH reduces bacterial activity)
  • Soil moisture is adequate (40–60% field capacity)
  • Used in combination with organic matter additions (vermicompost, jeevamrutha)

What Is Azospirillum and How Does It Work?

Azospirillum (Azospirillum brasilense, A. lipoferum) is a free-living nitrogen-fixing bacterium that colonises the root zone and rhizosphere of cereal crops, vegetables, and grasses, fixing atmospheric nitrogen (Nβ‚‚) into ammonium (NH₄⁺) that plants absorb. Unlike Rhizobium (which fixes nitrogen only in legume root nodules), Azospirillum works on virtually all crops without forming nodules β€” it lives freely near roots and fixes nitrogen in the root zone.

Additional benefits beyond N fixation:

  • Produces plant growth hormones (IAA β€” indole acetic acid), increasing root length and root hair density
  • Increased root surface area improves water and nutrient absorption even beyond nitrogen
  • Produces siderophores that chelate iron and make it available to plants in iron-deficient soils

Crops that respond best to Azospirillum:

  • Cereals (paddy, maize, wheat, sorghum, finger millet) β€” 15–30% of nitrogen requirement covered
  • Vegetables (all types) β€” root growth stimulation; 10–20% N savings
  • Sugarcane β€” widely used in Karnataka; documented 10–25% yield increase

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How Do You Apply PSB and Azospirillum?

Application MethodRateTimingNotes
Seed treatment5 g per kg of seed for each productJust before sowing; treat seeds within 24 hours of applicationMoisten seeds lightly; sprinkle PSB and Azospirillum powders; mix to coat; dry in shade 30 minutes; sow immediately
Seedling root dip250 g each in 10 litres water; dip roots for 30 minutesAt transplanting β€” before placing seedling in transplant holeFor transplanted crops (tomato, brinjal, capsicum, chilli); combined with Trichoderma root dip is excellent
Soil application (broadcast)2–2.5 kg each per acre mixed with 100 kg vermicompost or FYMAt last ploughing or bed preparation; incorporate into top 15 cmMost effective if mixed with vermicompost; vermicompost provides carbon and protects microbes during soil application
Drip fertigation1 kg dissolved in 10 litres water; inject at 1:50 dilutionMonthly during crop season; early morning applicationFilter through fine cloth before injection; flush drip system after; do not use within 7 days of any copper spray

Can you apply PSB and Azospirillum together? Yes β€” they are fully compatible and recommended together. Apply both in the same seed treatment or soil application.

What Is the Difference Between PSB, Azospirillum, and Rhizobium?

BiofertiliserMechanismCropsNotes
PSB (Phosphate Solubilising Bacteria)Solubilises locked soil phosphorusAll cropsNo crop specificity; works universally; most impactful in red laterite and acid soils
AzospirillumFree-living N fixation in root zone; root growth stimulationAll crops β€” most effective on cereals and vegetablesDoes not form nodules; works on all crops; 15–30% N savings documented
RhizobiumSymbiotic N fixation inside root nodulesLegumes only (bean, groundnut, soybean, cowpea, guar)Fixes 50–200 kg N/acre in legumes; completely ineffective on non-legumes; highly crop-specific strains
Mycorrhizae (AMF)Hyphal network extends root reach; phosphorus and water uptakeAll non-brassica cropsNot a N or P producer β€” extends access to existing soil P and water; synergistic with PSB
TrichodermaBiological fungicide; root zone disease suppressionAll cropsNot a nutrient fixer β€” pathogen control only; compatible with all biofertilisers

How Do You Store and Handle Biofertiliser Products?

PSB and Azospirillum are perishable living products β€” storage conditions determine effectiveness:

  • Ideal storage: 4–10Β°C (refrigerator is adequate); never freeze
  • Shelf life: 6–12 months from manufacture date; check the expiry date on every packet
  • Never store in direct sunlight or in a vehicle in summer β€” 30 minutes at 45Β°C can kill 80% of viable cells
  • After opening, seal tightly and use within 1 month
  • Quality check: good PSB smells earthy; Azospirillum product has a slight musty fermented smell; if it smells putrid (rotten egg / strong ammonia), the product is dead

Mix Biofertilisers with Jeevamrutha for a Complete Soil Drench β€” One Application, Multiple Benefits

A powerful soil drench combines Jeevamrutha (microbial inoculant, growth factors) with PSB and Azospirillum dissolved in it: prepare 200 litres Jeevamrutha as usual; add 500 grams each of PSB and Azospirillum powder; mix and apply as soil drench at 200 litres per acre. This single application delivers native soil microbes (from Jeevamrutha), phosphorus-solubilising bacteria, and nitrogen-fixing bacteria simultaneously. The Jeevamrutha provides the carbon, moisture, and microbial community that supports all three. In Karnataka’s laterite soil conditions β€” where phosphorus locking is acute β€” this combination addresses two major soil deficiencies in one step. Cost for the combined treatment: β‚Ή300–500 per acre vs β‚Ή3,000–6,000 for chemical NPK equivalents.

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Last updated: March 2026

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Related Guides

Jeevamrutha Preparation β†’ Trichoderma Guide β†’ Soil Biology Mycorrhizae β†’ Bio Inputs Application Schedule β†’ Seed Treatment Methods β†’

Last updated: March 2026

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